There are two types of assays available:
- SensiScreen® Ready-to-use CE-IVD Assays: SensiScreen® Ready-to-use assays are pre-dispensed in PCR strips and require only addition of patient DNA for somatic mutation testing.
- SensiScreen® Dispense Ready CE-IVD Mixes: SensiScreen® Dispense Ready CE-IVD Mixes include all reagents for somatic mutation testing except plastic ware to be used with the real-time PCR system of choice.
Overview of mutations detected by SensiScreen®
SensiScreen® assays detect the most common somatic mutations present in KRAS, NRAS, BRAF and EGFR. Furthermore, a range of new assays is expected to be added to the portfolio in 2017.
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NRAS mutations detected by SensiScreen® |
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| Assay | CDS mutation | Amino acid mutation | Cosmic ID |
| NRAS exon 2 | c.35G>C | Gly12Ala (G12A) | COSM565 |
| c.34G>T | Gly12Cys (G12C) | COSM562 | |
| c.35G>A | Gly12Asp (G12D) | COSM564 | |
| c.34G>C | Gly12Arg (G12R) | COSM561 | |
| c.34G>A | Gly12Ser (G12S) | COSM563 | |
| c.35G>T | Gly12Val (G12V) | COSM566 | |
| c.38G>C | Gly13Ala (G13A) | COSM575 | |
| c.37G>T | Gly13Cys (G13C) | COSM570 | |
| c.38G>A | Gly13Asp (G13D) | COSM573 | |
| c.37G>C | Gly13Arg (G13R) | COSM569 | |
| c.37G>A | Gly13Ser (G13S) | COSM571 | |
| c.38G>T | Gly13Val (G13V) | COSM574 | |
| NRAS exon 3 | c.183A>T | Gln61His (Q61H1) | COSM585 |
| c.183A>C | Gln61His (Q61H2) | COSM586 | |
| c.181C>A | Gln61Lys (Q61K) | COSM580 | |
| c.182A>T | Gln61Leu (Q61L) | COSM583 | |
| c.182A>G | Gln61Arg (Q61R) | COSM584 | |
| c.176C>A | Ala59Asp (A59D) | COSM253327 | |
| c.175G>A | Ala59Thr (A59T) | COSM578 | |
| NRAS exon 4 | c.351G>C | Lys117Asn (K117N1) | N/A |
| c.351G>T | Lys117Asn (K117N2) | N/A | |
| c.436G>C | Ala146Pro (A146P) | (COSM4172577) | |
| c.436G>A | Ala146Thr (A146T) | COSM27174 | |
| c.437C>T | Ala146Val (A146V) | COSM4170228 | |