The Randomly Amplified Polymorphic DNA (RAPD) method has been successfully applied to the identification of many bacterial strains. This fast and sensitive method is able to provide reproducible and characteristic fingerprints of complex genomes without prior sequence information. The use of short primers of arbitrary sequence during PCR results in amplification of different segments of genomic DNA, which after gel electrophoresis give rise to characteristic band patterns. Most informative DNA bands are usually in the 300-3000bp range.
To cover the relatively vast range of different fragment sizes, Elchrom Scientific recommends to use Poly(NAT)® gels.
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