The Single Strand Conformational Polymorphism (SSCP) method relies on different migration rates of single stranded DNA fragments in a non-denaturing gel. Two single stranded DNA fragments whose sequence differs in just one nucleotide often migrate at sufficiently different rates to allow separation of the two molecules. The success of an SSCP analysis critically depends on constant low temperature during electrophoresis.
Our GMA-Gels (Gene Mutation Analysis) gels have been optimized for detecting DNA mutations by SSCP and Heteroduplex in parallel.
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Heteroduplex analysis is a mutation scanning method to identify duplex DNA formed by strands from different sources. Sequence variations can cause loops and bubbles or even alter the basic structure of the helix in regions where the two DNAs differ, which has an impact on the mobility within a gel matrix. Due to the non-denaturing structure of our matrix, GMA is perfectly suited to achieve reliable results for heteroduplex analysis and SSCP in parallel.
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